The wind turbine (WT) generally provides auxiliary frequency regulation through integrated inertia control, but its frequency regulation effect is greatly affected by control parameters. In this paper, the tuning of frequency control parameters is studied by considering the adjustable ability of the WT and the system frequency response index. Firstly, a system frequency response (SFR) model is established, which takes into account the participation of wind power and thermal power in frequency regulation, and the analytic expression of the frequency response indices is derived. Secondly, after linearizing the rotor motion equation of the WT, the rotor speed model is established, and its accuracy is verified under different wind speed conditions. Then, the control parameters of the WT are set up with the aim of raising the frequency nadir and considering the rotor speed limit and the safety constraint of the system frequency. Finally, the simulation results verify that the proposed frequency control parameter tuning method can fully utilize the rotor kinetic energy of the WT under the premise of ensuring the safety of the system frequency.